The round-eyed slow loris is reported to have an extremely high mortality rate in captivity and is on the list of endangered species.
Suchai Jalernphu, 30, and Natchapon Kensing, 20, were charged with possession of protected native animals.
While the two adults and a juvenile loris were exceedingly photogenic, horrified tourists are believed to have tipped off police to the presence of the nocturnal animals and their handlers in Soi Bangla.
The police stepped in and arrested the men about 8pm.
The lorises, native to Southeast Asia, were taken to the Gibbon Sanctuary in Bang Pae, eastern Phuket, where they will be checked to ensure they are healthy before release into the wild.